EasySeq™ NGS 文库构建系列EasySeq™ NGS Library Preparation Series
RC-PCR®（Reverse Complement PCR）是NimaGen的核心专利技术。
RC-PCR® (Reverse Complement PCR) is NimaGen's core patented technology.
该技术将目标扩增、接头连接及**双索引（UDI）添加整合至单管PCR反应中，在封闭体系内完成整个扩增子文库构建流程。
This technology integrates target amplification, adapter ligation, and unique dual indexing (UDI) into a single tube PCR reaction, completing the entire amplicon library preparation workflow in a closed system.
EasySeq™ NGS文库构建系列基于 RC-PCR® 技术开发，面向人类遗传学、微生物组学及公共卫生检测应用。
The EasySeq™ NGS Library Preparation Series is developed based on RC-PCR® technology for applications in human genetics, microbiome research, and public health testing.
同时，NimaGen采用 RC-PCR® 方法开发了用于 Oxford Nanopore Technologies® 平台的全长16s文库制备解决方案（Full-length 16S Library Prep Kit），对细菌16S基因进行全面分析，可实现物种级别的鉴定，并能在8小时内完成从样本到结果的全过程。
In addition, NimaGen has used the RC-PCR® approach to develop a full length 16S Library Prep Kit for the Oxford Nanopore Technologies® platform. It enables comprehensive analysis of the bacterial 16S gene, allowing species level identification, and can complete the entire process from sample to result within 8 hours.
产品/Products:
产品特点/Product Features:
操作简便，减少人为误差 Simple operation, reducing manual errors
适配多种主流测序平台Compatible with multiple major sequencing platforms
单管操作，显著降低污染风险Single‑tube workflow significantly reduces contamination risk
无需二次PCR与中间纯化步骤No need for secondary PCR or intermediate purification steps
支持低起始量DNA样本Supports low‑input DNA samples
有效减少Index Hopping Effectively reduces index hopping
提升建库效率与数据准确性Improves library preparation efficiency and data accuracy
典型应用包括/Typical applications include:
人类遗传病相关基因检测Genetic testing for human hereditary diseases
样本身份验证与追踪Sample identity verification and tracking
16S 微生物组分析16S microbiome analysis
传染病监测与分型Infectious disease surveillance and typing
EasySeq™ 将建库流程标准化，帮助实验室实现更高通量与更稳定的数据输出。
EasySeq™ standardizes the library preparation process, helping laboratories achieve higher throughput and more consistent data output.
