500 bp 的双 gRNA 变体的多基因片段库显示片段均一性以及*小的丢失（图 1），450 bp 的三重 gRNA 变体库亦是如此（图 2）。*后，多基因片段库中GC含量范围很广，包括GC高达80%的片段，显示出高度均一性和*小的偏好（图3）。
Figure 1. Graph of read count of each of the mapped dual gRNA variants (X-axis) for an amplified pool of Multiplexed Gene Fragments of 500 bp.
Figure 2. Graph of read count of each of the mapped triple gRNA variants (X-axis) for an amplified pool of Multiplexed Gene Fragments of 450 bp.
Figure 3. GC plots of an amplified pool of Multiplexed Gene Fragments of 500 bp length were created and show minimal bias among a wide range of GC content up until 80%. X-axis are categories of 14 groups of sequences with different GC content. Y-axis is the read count.
